| CatalogCode: | NB120-3297 |
| ProductName: | Laminin B2 / gamma 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Laminin B2 / gamma 1 (D18) |
| Clone: | D18 |
| Clonality: | Monoclonal |
| Immunogen: | Rat glomerular basement membrane. |
| CrossReactivity: | Cross-reacts with Human, Rat and Guinea pig. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | For staining of formalin fixed tissues, digest sections with Protease XXV at 1mg / ml PBS for 5 minutes at 37 degrees C. |
| Localization: | Basement membrane. |
| Control: | LS174T cells. Skin tissue sections. |
| Background: | Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin / nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2a kappa |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | 295 |
| AppSummary: | IHC-P |
| SpeciesSummary: | Hu, Rt, Gp |
| ProteinTarget: | Laminin B2 / gamma 1 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Green TL et al. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem 267:2014-22 (1992). Sanes JR et al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990). |