| CatalogCode: | NB120-3290 |
| ProductName: | Plastin L Antibody |
| Product Description: | Mouse Monoclonal anti-Plastin L (LPL4A.1) |
| Clone: | LPL4A.1 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Epitope: | Headpiece region. |
| Specificity: | This antibody does not react with T or I plastin.It recognizes LPL in Calcium independent fashion. |
| CrossReactivity: | Cross-reacts with Human.Does not cross-react with Mouse.Not yet tested in other species. |
| Packaging: | 0.5 ml Liquid. |
| Uses: | Immunofluorescence (fixed in methanol or 3%paraformaldehyde): assay dependent.Western Blotting: use at a concentration of 1 - 2ug / ml for 2h at RT.Immunoprecipitation (native, with protein G): use at a concentration of 2ug / mg of protein lysate (Lollike K, et al.).Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Plastin L. |
| Localization: | Cytoplasmic. |
| Control: | Human polymorphonuclear neutrophils / monocytes, MCF 7, and Jurkat cells. |
| Background: | L plastin is a leukocyte specific actin bundling protein from the fimbrin family of proteins. It has two actin binding domains and two EF hand type calcium binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L plastin may play a role in regulating integrin mediated adhesion in leukocytes. L plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosterone in androgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 330 |
| AppSummary: | IF, IP, WB |
| SpeciesSummary: | Hu, Mu (-) |
| ProteinTarget: | Plastin L |
| PackageSize: | 0.5 ml |
| GeneralRef: | This product has been used in:Lollike K et al. Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner. J Biol Chem 276:17762-9 (2001). General / background references:Jones SL & Brown EJ FcgammaRII-mediated adhesion and phagocytosis induce L-plastin phosphorylation in human neutrophils. J Biol Chem 271:14623-30 (1996). |
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