| CatalogCode: | NB120-3289 |
| ProductName: | Laminin S Antibody |
| Product Description: | Mouse Monoclonal anti-Laminin S (C4) |
| Clone: | C4 |
| Clonality: | Monoclonal |
| Immunogen: | Bovine anterior lens capsule. |
| CrossReactivity: | Cross-reacts with Human, Rat, Cow, Pig, Guinea pig and Chicken. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Immunoprecipitation use with Protein G. Western Blotting: use for 2hrs at RT. By Western blot, this antibody detects a band of 190 kDa, which corresponds to the predicted molecular weight of Laminin S. This antibody is not suitable for immunohistochemistry. |
| Localization: | Basement membrane. |
| Control: | LS174T cells. |
| Background: | Extracellular matrix (ECM) surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Dystroglycan is a major laminin receptor in muscle and epithelia; and nidogen is a high affinity laminin binding protein important for basement membrane assembly. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | 325 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Bv, Ch, Hu, Po, Rt, Gp |
| ALTnames: | Anti-LAMB2 antibody; Anti-Laminin B1s chain antibody; Anti-Laminin subunit beta 2 antibody; Anti-LAMS antibody; Anti-S laminin antibody |
| ProteinTarget: | Laminin S |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Zwi LJ et al. Visualization of blood vessels without prior perfusion. Microvasc Res 58:79-82 (1999). Burgi-Saville ME et al. Expression patterns of extracellular matrix components in native and cultured normal human thyroid tissue and in human toxic adenoma tissue. Thyroid 7:347-56 (1997). Maier A & Mayne R Basal lamina development in chicken muscle spindles. Dev Dyn 202:284-93 (1995). Green TL et al. Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J Biol Chem 267:2014-22 (1992). Sanes JR et al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990). Hunter DD et al. A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction. Nature 338:229-34 (1989). Sanes JR et al. Cold Spring Harbor Symposia on Quantitative Biology XLVIII 667-678 (1983). |
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