| CatalogCode: | NB120-3286 |
| ProductName: | Hsp 75 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp 75 (TRAP1-6) |
| Clone: | TRAP1-6 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant TRAP 1 protein. |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml Liquid. |
| Uses: | Western Blotting: use at a concentration of 5ug / ml for 2hrs at RT.Not suitable for immunopreciptation.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 75 kDa, which corresponds to the predicted molecular weight of Hsp 75. |
| Localization: | Cytoplasmic. |
| Control: | SKBR 3, A431 or HeLa cell lysate. |
| Background: | A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSP's), more appropriately called as stress response proteins (SRP's). The Hsp 90 family of proteins in mammalian cells consists of Hsp 90 alpha and beta, Grp 94, and TRAP 1 (Hsp 75). It was found that human TRAP 1 is localized to mitochondria and exhibited ATPase activity that was inhibited by both geldanamycin and radicicol indicating that TRAP 1 has functions that are distinct from those of hsp 90. It was also found that TRAP 1 had a biochemicalactivity to refold denatured retinoblastoma protein (Rb) into its native conformation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein G purified |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 330 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | Hsp 75 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Felts SJ et al. The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties. J Biol Chem 275:3305-12 (2000). |