| CatalogCode: | NB120-3267 |
| ProductName: | Rhodopsin Antibody |
| Product Description: | Mouse Monoclonal anti-Rhodopsin (RET-P1) |
| Clone: | RET-P1 |
| Clonality: | Monoclonal |
| Immunogen: | Membrane preparation from adult rat retina. |
| Specificity: | This antibody specifically labels the axons andsynaptic pedicles of the rods. |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Immunofluorescence: assay dependent. Western Blotting: use at a concentration of 1 - 2ug / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4ug / ml for 30 min at RT. By Western blot, this antibody detects a band of 39 kDa, which corresponds to the predicted molecular weight of Rhodopsin. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. |
| Localization: | Plasma membrane |
| Control: | IMR-5 cellsBrain or retina tissue sections. |
| Background: | Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | 325 |
| AppSummary: | IF, WB, IHC-P |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-OPN 2 antibody; anti-Opsin 2 antibody; anti-Retinitis Pigmentosa 4 antibody; anti-RHO antibody; anti-RP 4 antibody |
| ProteinTarget: | Rhodopsin |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Treisman JE et al. Opsin expression in the rat retina is developmentally regulated by transcriptional activation. Mol Cell Biol 8:1570-9 (1988). Hicks D & Barnstable CJ Different rhodopsin monoclonal antibodies reveal different binding patterns on developing and adult rat retina. J Histochem Cytochem 35:1317-28 (1987). Fekete DM & Barnstable CJ The subcellular localization of rat photoreceptor-specific antigens. J Neurocytol 12:785-803 (1983). Barnstable CJ Monoclonal antibodies which recognize different cell types in the rat retina. Nature 286:231-5 (1980). |
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