| CatalogCode: | NB120-3250 |
| ProductName: | Caspase 9 Antibody |
| Product Description: | Mouse Monoclonal anti-Caspase 9 (9CSP03 (96.1.23)) |
| Clone: | 9CSP03 (96.1.23) |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant human Caspase 9 prodomain. |
| Epitope: | Caspase 9 prodomain. |
| CrossReactivity: | Cross-reacts with Human and Mouse.Does not cross-react with Rat.Not yet tested in other species. |
| Packaging: | 0.5 ml Liquid. |
| Uses: | Immunoprecipitation (native and denatured, with protein G): use at a concentration of 2ug / mg of lysate.Western Blotting: use at a concentration of 1 - 2ug / ml for 2hrs at RT.Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2ug / ml for 30 min at RT.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 46 - 48 kDa, which corresponds to the predicted molecular weight of Caspase 9.Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Control: | Jurkat cells. Tonsil tissue sections. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein G purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 330 |
| AppSummary: | IHC-P, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti-APAF 3 antibody, anti-APAF3 antibody, anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic protease activating factor 3 antibody, anti-Apoptotic protease Mch 6 antibody, anti-CASP 9 antibody, anti-ICE LAP6 antibody, anti-ICE like apoptotic protease 6 APAF 3 antibody, anti-APAF3 antibody, anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic protease activating factor 3 antibody, anti-Apoptotic protease Mch 6 antibody, anti-CASP 9 antibody, anti-ICE LAP6 antibody, anti-ICE like apoptotic protease 6 antibody |
| ProteinTarget: | Caspase 9 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Hakem R et al. Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94:339-52 (1998). Li P et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-89 (1997). |
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