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| Catalog Code: | NB120-3227 |
| Product Name: | c-Abl Antibody |
| Product Description: | Mouse Monoclonal anti-c-Abl (8E9) |
| Clone: | 8E9 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Recombinant full length protein. |
| Epitope: | SH2 domain. |
| Specificity: | NB 120-3227 reacts with c-Abl p120 and chimeric Bcr / Abl proteins. |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Western Blotting: use for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use for 30min at RT.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 120 kDa, which corresponds to the predicted molecular weight of c-Abl.Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | The c-Abl proto oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr / Abl mRNA encoding activated Abl protein tyrosine kinase. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 lambda |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, |
| Application Summary: | immunofluorescence , immunoprecipitation 2ug/mg, Western Blot 1ug/ml, Immunohistochemistry-Paraffin 2 - 4ug/ml |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-Abelson Murine Leukemia Viral Oncogene Homolog 1 antibody, anti-Abelson murine leukemia viral v abl oncogene homolog 1 antibody, anti-Abl 1 antibody, anti-ABL antibody, anti-Abl protein antibody, anti-Abl1 antibody, anti-Bcr/c abl oncogene protein antibody, anti-JTK 7 antibody, anti-JTK7 antibody, anti-p150 antibody, anti-Proto oncogene tyrosine protein kinase ABL1 antibody, anti-Transformation gene oncogene ABL antibody, anti-v abl Abelson murine leukemia viral oncogene homolog 1 antibody, anti-v abl antibody |
| Package Size: | 0.5 ml |
| Preservative: | Sodium Azide |
| General References: | General / background references:Whang YE et al. c-Abl is required for development and optimal cell proliferation in the context of p53 deficiency. Proc Natl Acad Sci U S A 97:5486-91 (2000). <PUBMED:10805805>Sun X et al. Activation of the cytoplasmic c-Abl tyrosine kinase by reactive oxygen species. J Biol Chem 275:17237-40 (2000). <PUBMED:10770918> |
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more information: | company product webpage |