| Catalog Code: | NB120-3215 |
| Product Type: | Primary Antibody |
| Product Name: | Nck Antibody |
| List Price: | 330 |
| Clonality: | Monoclonal |
| Gene ID: | 4690 |
| Host: | Mouse |
| Clone: | NC-20 (same as 20B-1H9) |
| Isotype: | IgG1 kappa |
| Product Description: | Mouse Monoclonal anti-Nck (NC-20 (same as 20B-1H9)) |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Species Summary: | Hu |
| Background: | Nck is a highly conserved protein that has one SH2 and three SH3 domains. It is ubiquitously expressed in mammalian tissues. Nck is phosphorylated on tyrosine, serine and threonine residues in response to stimulation by EGF and PDGF. Nck has been proposed |
| Immunogen: | Recombinant full length protein (Human). |
| Localization: | Cytoplasmic |
| Purity: | Protein G purified |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| Packaging: | 500 microlitres Liquid. |
| Package Size: | 0.5 ml |
| Uses: | Immunoprecipitation (native and denatured): use at a concentration of 2ug / mg.Western Blotting: use at a concentration of 2 - 4ug / ml for 2hrs at RT.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user |
| Application Summary: | IP, WB |
| General References: | General / background references:Kitamura Y et al. Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac. Biochem J 322 (Pt 3):873-8 (1997). Birge RB et al. SH2 and SH3-containing adaptor proteins: redundant or inde |