| CatalogCode: | NB120-3193 |
| ProductName: | Bcl-x Antibody |
| Product Description: | Mouse Monoclonal anti-Bcl-x (2H12) |
| Clone: | 2H12 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. This amino acid sequence is shared by human and murine bcl-X protein. |
| Epitope: | aa 3 - 14 |
| Specificity: | ab3193 reacts with both Bcl-XS and Bcl-XL proteins. |
| CrossReactivity: | Cross-reacts with Human, Rat, Mouse and Pig.Not yet tested in other species. |
| Packaging: | 0.5 ml Liquid. |
| Uses: | Flow Cytometry / Immunofluorescence: assay dependent.Immunoprecipitation (native): use at a concentration of 2ug / mg.Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4ug / ml for 30 min at RT.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Localization: | Mitochondrial membranes and perinuclear envelope |
| Control: | Hodgkin's lymphoma |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein A purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 330 |
| AppSummary: | FACS, IF, IHC-P, IP |
| SpeciesSummary: | Hu, Mu, Rt, Po |
| ProteinTarget: | Bcl-x |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Hsu YT & Youle RJ Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 272:13829-34 (1997). Hsu YT et al. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A 94:3668-72 (1997). Wolter KG et al. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139:1281-92 (1997). |
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