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| Catalog Code: | NB120-3117 |
| Product Name: | Cytokeratin Type 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin Type 1 (AE1) |
| Clone: | AE1 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Human epidermal keratin. |
| Specificity: | This antibody reacts with MCF-7, RT 112 and A 431 cultured cell lines. |
| Cross Reactivity: | Cross-reacts with Human, Rabbit, Rat, Mouse, Cow, Chicken and Monkey. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37 degrees C or Protease XXV at 1mg/ml PBS for 5minutes at 37 degrees C. This antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. |
| Localization: | Cytoplasmic. |
| Background: | Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65 - 67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| Application Summary: | Immunohistochemistry-Paraffin 1-2 ug/ml for 30 min at RT, Immunohistochemistry-Frozen Use at an assay dependent dilution, immunofluorescence Use at an assay dependent dilution, Immunocytochemistry, |
| Species Summary: | Bv, Ch, Hu, Mu, Mk, Rt, Rb, |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | General / background references:Eichner R et al. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. J Cell Biol 98:1388-96 (1984). Cooper D et al. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation 28:30-5 (1984). Woodcock-Mitchell J et al. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580-8 (1982). Tseng SC et al. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell 30:361-72 (1982). |
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