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| Catalog Code: | NB120-3104 |
| Product Name: | c-erbB-4 Antibody |
| Product Description: | Mouse Monoclonal anti-c-erbB-4 (H4.77.16) |
| Clone: | H4.77.16 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Extracellular fragment of recombinant human c-erbB-4 / HER 4 oncoprotein. |
| Epitope: | Extracellular domain. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Flow Cytometry: assay dependent.Functional studies (see below): assay dependent.Immunoprecipitation (with protein G): use at a concentration of 2ug/mg of protein lysate.This antibody is not suitable for western blot (Tang C, et. al).This antibody does not interfere with receptor-heregulin binding and is particularly wellsuited for immunoprecipitation of unoccupied as wellas the occupied c-erbB-4 receptor (Chen X, et. al.). It reacts withintact cells, stimulates tyrosine kinase activity, andefficiently stimulates internalization of c-erbB-4 (Chen X, et. al.). |
| Localization: | Type I membrane protein |
| Background: | C-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| Application Summary: | flow cytometry / FACS analysis, immunoprecipitation, Other |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-AP1 antibody, anti-V-Jun antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | General / background references:Tang CK et al. Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo. Cancer Res 59:5315-22 (1999). Chen X et al. An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4. J Biol Chem 271:7620-9 (1996). |
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