| CatalogCode: | NB120-3092 |
| ProductName: | Collagen II Antibody |
| Product Description: | Mouse Monoclonal anti-Collagen II (5B2.5) |
| Clone: | 5B2.5 |
| Clonality: | Monoclonal |
| Immunogen: | A purified preparation of lathyritic type II collagen from embryonic chicken sternum. |
| Epitope: | GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues consitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic type II collagen with recombinant stromelysin. |
| Specificity: | This antibody is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, this antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Chicken. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | Not suitable for IHC. |
| Localization: | Cartilage matrix. |
| Control: | Cartilage. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2a kappa |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | 295 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Ch, Hu, Mu |
| ProteinTarget: | Collagen II |
| PackageSize: | 0.5 ml |
| GeneralRef: | This product has been used in:Mayne R et al. Connect Tissue Res. 31:11-21 (1994).General / background references:Chen Q et al. Type II collagen during cartilage and corneal development: immunohistochemical analysis with an anti-telopeptide antibody. Dev Dyn 196:47-53 (1993). |
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