| Catalog Code: | NB120-300 |
| Product Type: | Primary Antibody |
| Product Name: | Collagen Type II Antibody |
| List Price: | 330 |
| Clonality: | Polyclonal |
| Gene ID: | 1280 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-Collagen Type II |
| Cross Reactivity: | Cross-reacts with Cow and Human.Expected to cross-react with most Mammals due to sequence homology.Not yet tested in other species. |
| Species Summary: | Bv, Hu |
| Background: | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate an |
| Immunogen: | Collagen Type II from adult human knee cartilage and bovine nasal cartilage. |
| Specificity: | This antibody has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Purity: | Immunogen affinity purified |
| Buffer: | 125mM sodium borate, 75mM sodium chloride, 5mM EDTA, pH 8.0 with 0.01% sodium azide |
| Packaging: | 100 micrograms Liquid (sterile filtered). |
| Package Size: | 0.1 mg |
| Uses: | Suggested working dilutions: Western blotting (non-denaturing, non-dissociating): 1:5,000-1:10,000, Indirect ELISA (against 1.0ug collagen II): 1:4,000-1:8,000 Histochemistry (frozen sections): 1:50-1:200.Optimal titers for other applications should be d |
| Application Summary: | ELISA, IHC-Fr, IP, WB |