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| Catalog Code: | NB120-2982 |
| Product Name: | Catenin (beta) Antibody |
| Product Description: | Rabbit Polyclonal anti-Catenin (beta) |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Recombinant full length protein (Human). |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Dog. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Rabbit antisera. |
| Uses: | Immunofluorescence: assay dependent.Immunoprecipitation (with protein A, native): use at a concentration of 10ug / mg of lysate.Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 10 - 20 ug / ml for 30 min at RT.Western Blotting: use at a concentration of 2.5 - 5ug / ml for 2hrs at RT. By Western blot, this antibody detects a band of ~ 92 kDa, which corresponds to the predicted molecular weight of beta Catenin.Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. |
| Localization: | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Storage: | The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development. |
| Purity: | Store at 4C. Do not freeze. |
| Isotype: | protein A purified |
| Host Name: | IgG |
| Buffer: | Rabbit |
| Application Summary: | 10mM PBS, pH7.4, 0.2%BSA |
| Species Summary: | immunofluorescence , immunoprecipitation 10ug/mg , Western Blot 2.5 - 5ug/ml, Immunohistochemistry-Paraffin 10 - 20 ug/ml |
| Alternate Names: | Ca, Hu, Mu, |
| Package Size: | Anti-Cadherin associated protein antibody; Anti-Catenin beta 1 antibody; Anti-CATNB antibody; Anti-CTNNB antibody; Anti-CTNNB1 antibody |
| Preservative: | 0.5 ml |
| Homology: | 0.09% sodium azide |
| Product References: | General / background references:Roe S et al. A mutation in alpha-catenin disrupts adhesion in clone A cells without perturbing its actin and beta-catenin binding activity. Cell Adhes Commun 5:283-96 (1998). Koslov ER et al. Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin. J Biol Chem 272:27301-6 (1997). Hazan RB et al. Vinculin is associated with the E-cadherin adhesion complex. J Biol Chem 272:32448-53 (1997). Rimm DL et al. Reduced alpha-catenin and E-cadherin expression in breast cancer. Lab Invest 72:506-12 (1995). |
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