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| Catalog Code: | NB120-2964 |
| Product Name: | Cullin 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cullin 1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: C-DGEKDTYSYLA, corresponding to amino acids 742-752 of Human Cullin 1. |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Does not cross-react with Rat. Not yet tested in other species. |
| Packaging: | 0.05 mg Rabbit antisera. |
| Uses: | Immunoprecipitation, Western Blotting, Immunohistochemistry (formalin fixed paraffin embedded)Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 86 kDa, which corresponds to the predicted molecular weight of Cullin 1.No special pretreatment is required for immuno histochemical staining of formalin fixed tissues. |
| Background: | Cullin-1 is a member of the family of human cullin genes (cullin-1, 2, 3, 4a, 4b, and 5) homologous to the s. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2. Both of which, together with cdc53, as three subunits, form an ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The cullin-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| Application Summary: | immunohistochemistry , immunoprecipitation Ab 10 ìg/mg protein lysate, Western Blot 1 to 2 ug/ml, Immunohistochemistry-Paraffin |
| Species Summary: | Hu, Mu, Rt(-), , |
| Alternate Names: | anti-CUL 1 antibody, anti-CUL1 antibody,anti-Cullin1 antibody, anti-MGC149834 antibody, anti-MGC149835 antibody |
| Package Size: | 0.05 mg |
| General References: | General / background references:Michel JJ & Xiong Y Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ 9:435-49 (1998). Pagano M Cell cycle regulation by the ubiquitin pathway. FASEB J 11:1067-75 (1997). Kipreos ET et al. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85:829-39 (1996). |
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