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| Catalog Code: | NB120-2955 |
| Product Name: | Cdk 8 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdk 8 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human Cdk8. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Rabbit antisera. |
| Uses: | Western Blotting: use at 2hrs at RT. By Western blot, this antibody detects a band of 54 kDa, which corresponds to the predicted molecular weight of Cdk 8. |
| Localization: | Nuclear and cytoplasmic. |
| Background: | A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34 / Cdc2, and a related protein Cdk2, function late in the cycle while Cdk4 and Cdk6 are critically involved in G1 to S progression. p54 / Cdk8 interacts exclusively with CyclinC. The Cdk8-CyclinC complex activates the kinase. Because of analogy with yeast Cdk8, it has been proposed that the human Cdk8-CyclinC may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA |
| Application Summary: | immunofluorescence , immunoprecipitation 10ug /mg of lysate, Western Blot 1-2ug/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-Cell Division Kinase 8 antibody; Anti-Cyclin Dependent kinase 8 antibody; Anti-K35 antibody; Anti-Protein kinase K35 antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | General / background references:Tassan JP et al. Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C. Proc Natl Acad Sci U S A 92:8871-5 (1995). |
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