| CatalogCode: | | NB120-2949 |
| ProductName: | | Cyclin B1 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Cyclin B1 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (Human). |
| CrossReactivity: | | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | | 0.5 ml Affinity purified Rabbit antisera. |
| Uses: | | Immunoprecipitation use with protein A, native and denatured. Immunohistochemistry (formalin fixed paraffin embedded): use for 30 min at RT. By Western blot, this antibody detects a band of 62 kDa, which corresponds to the predicted molecular weight of Cyclin B1. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. |
| Localization: | | Inactive cyclin B cytoplasmic. |
| Control: | | Jurkat, Ls174T or HeLa cells. Breast carcinoma tissue section. |
| Background: | | In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | Affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 10mM PBS, pH7.4, 0.2%BSA |
| ListPrice: | | 325 |
| AppSummary: | | IP, WB, IHC-P |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-CCNB 1 antibody; anti-CCNB antibody; anti-CCNB1 antibody; anti-G2 mitotic specific cyclin B1 antibody |
| ProteinTarget: | | Cyclin B1 |
| PackageSize: | | 0.5 ml |
| GeneralRef: | | General / background references:Baroja A et al. Genesis and evolution of high-ploidy tumour cells evaluated by means of the proliferation markers p34(cdc2), cyclin B1, PCNA and 3[H]-thymidine. Cell Prolif 29:89-100 (1996). Brandeis M & Hunt T The proteolysis of mitotic cyclins in mammalian cells persists from the end of mitosis until the onset of S phase. EMBO J 15:5280-9 (1996). David-Pfeuty T & Nouvian-Dooghe Y Human cyclin B1 is targeted to the nucleus in G1 phase prior to its accumulation in the cytoplasm. Oncogene 13:1447-60 (1996). |
| more information: | | company product webpage |
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