| CatalogCode: | NB120-2947 |
| ProductName: | p27 / KIP 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-p27 / KIP 1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: VEQTPKKPGLRRQT, corresponding to amino acids 184-197 of Mouse p27 KIP 1. |
| CrossReactivity: | Cross-reacts with Mouse.Does not cross-react with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml Liquid. |
| Uses: | Immunoprecipitation (with protein A, natured and denatured): use at a concentration of 10ug / mg of lysate (co-precipitates cdk 4).Western Blotting: use at a concentration of 5 - 10ug / ml for 2 hrs at RT.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 27 kDa, which corresponds to the predicted molecular weight of p27 / KIP 1. |
| Localization: | Nuclear |
| Control: | MAD109 cells |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
| ListPrice: | 330 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Mu, Hu (-) |
| ProteinTarget: | p27 / KIP 1 |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Bullrich F et al. Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer. Cancer Res 55:1199-205 (1995). Chan FK et al. Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol 15:2682-8 (1995). Toyoshima H & Hunter T p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell 78:67-74 (1994). Ajchenbaum F et al. Independent regulation of human D-type cyclin gene expression during G1 phase in primary human T lymphocytes. J Biol Chem 268:4113-9 (1993). |
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