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| Catalog Code: | NB120-2939 |
| Product Name: | RANBP3 Antibody |
| Product Description: | Rabbit Polyclonal anti-RANBP3 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: MADLANEEKPAIA-C, corresponding to amino acids 1-13 of Human RanBP3. |
| Specificity: | Detects Ran binding protein 3 (RanBP 3) from human and rat cells. |
| Cross Reactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.2 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Immunofluorescence: use at a concentration of 10 ug/ml. Western Blot: use at a concentration of 10 ug/ml.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot this antibody detects an ~60 kDa protein representing RanBP 3 from HeLa cell extracts. Immunofluorescence staining of RanBP 3 in NRK cells results in diffuse nuclear staining. |
| Background: | Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane. RanBP3 functions as a cofactor for CRM1-mediated nuclear export and stimulates export by enhancing the affinity of CRM1 for RanGTP and substrate. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunofluorescence 10 ug/ml, Western Blot 10 ug/ml |
| Species Summary: | Hu, Rt |
| Package Size: | 0.2 mg |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | General / background references:Fauser S et al. Genomic organization, expression, and localization of murine Ran-binding protein 2 (RanBP2) gene. Mamm Genome 12:406-15 (2001). Ferreira PA Characterization of RanBP2-associated molecular components in neuroretina. Methods Enzymol 315:455-68 (2000). Chook YM et al. Pictures in cell biology. Structures of nuclear-transport components. Trends Cell Biol 9:310-1 (1999). |
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