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| Catalog Code: | NB120-2928 |
| Product Name: | Hsp 86 Antibody |
| Product Description: | Rabbit Polyclonal anti-Hsp 86 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: P(2)EETQTQDQPM(12). Immunizing peptide corresponds to amino acid residues 2-12 from the N-terminal mouse HSP 86 protein.The N-terminal regions of HSP 84 and HSP 86 show the largest difference in amino acid sequence. |
| Specificity: | Detects Heat Shock Protein 86 (HSP 86). This antibody does not detect HSP 84. |
| Cross Reactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.1 mg Liquid. |
| Uses: | Immunofluorescence: use at a concentration of 10-15 ug/ml.Western Blot: use at a concentration of 0.5 ug/ml.Immunoprecipitation: assay dependent. Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~86 kDa protein representing HSP 86 from HeLa cell lysate.Immunofluorescence staining of HSP 86 in HeLa cells yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP 86 exists primarily as homodimers in HeLa cells. This antibody is capable of precipitating HSP 86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor. |
| Localization: | Cell Membrane and Nuclear |
| Background: | Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP 90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP 90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, Ah receptor, and some kinases. Studies have shown that murine HSP 90 exists as two forms, HSP 84 and HSP 86, coded by related but separate genes, with 86% homologous amino acid sequences. These forms are analogous to the two forms of human HSP 90, HSP 89 beta and HSP 89 alpha. In an unstressed mouse fibroblast, the basal level of HSP 84 is found to be double that of HSP 86. However, after heat shock, HSP 86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP 86, but not HSP 84, decreases. HSP 84 and HSP 86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunofluorescence 10-15 µg/ml, immunoprecipitation , Western Blot 0.5 µg/ml, Immunohistochemistry-Paraffin 5 µg/ml |
| Species Summary: | Hu, Rt |
| Alternate Names: | anti-FLJ31884 antibody, anti-Heat shock 90kD protein 1 alpha antibody, anti-Heat shock 90kD protein 1 alpha like 4 antibody, anti-Heat shock 90kD protein alpha like 4 antibody, anti-Heat shock 90kDa protein 1 alpha antibody, anti-Heat shock protein 90kDa alpha class A member 1 antibody, anti-Heat shock protein 90kDa alpha cytosolic class A member 1 antibody, anti-Heat shock protein HSP 90 alpha antibody, anti-Hsp 86 antibody, anti-Hsp 89 antibody, anti-Hsp 90 antibody, anti-HSP 90A antibody, anti-HSP 90N antibody, anti-Hsp89 antibody, anti-Hsp90 antibody, anti-HSP90A antibody, anti-HSP90AA1 antibody, anti-HSP90N antibody, anti-HSPC 1 antibody, anti-HSPC1 antibody, anti-HSPCA antibody, anti-HSPCAL 1 antibody, anti-HSPCAL 4 antibody, anti-HSPCAL1 antibody, anti-HSPCAL4 antibody, anti-HSPN antibody, anti-LAP 2 antibody, anti-LAP2 antibody, anti-Renal carcinoma antigen NY REN 38 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | This product has been used in:Perdew GH et al. Localization and characterization of the 86- and 84-kDa heat shock proteins in Hepa 1c1c7 cells. Exp Cell Res 209:350-6 (1993). General / background references:Chen HS & Perdew GH Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex. J Biol Chem 269:27554-8 (1994). Moore SK et al. Characterization of the mouse 84-kD heat shock protein gene family. DNA Cell Biol 9:387-400 (1990). Moore SK et al. Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary origins. J Biol Chem 264:5343-51 (1989). |
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