| CatalogCode: | | NB120-2927 |
| ProductName: | | Hsp 84 Antibody |
| Product Description: | | Rabbit Polyclonal anti-Hsp 84 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide: P(2)EEVHHGEEEVE(13). Immunizing peptide corresponds to amino acid residues 2-13 from mouse HSP 84 protein. The N-terminal regions of HSP 84 and HSP 86 show the largest difference in amino acid sequence. |
| Specificity: | | Detects heat shock protein 84 (HSP 84). This antibody does not detect HSP 86. |
| CrossReactivity: | | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | | 0.1 mg Liquid. |
| Uses: | | Immunofluorescence: use at a concentration of 10-15 ug/ml. Western Blot: use at a concentration of 0.5 ug/ml. Immunoprecipitation: assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~84 kDa protein representing HSP 84 from Hepa1 cell lysate.Immunofluorescence staining of HSP 84 in Hepa1 cells yields a pattern consistent with cytoplasmic staining.Immunoprecipitation experiments with this antibody suggest that HSP 84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP 84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor. |
| Localization: | | Cytoplasmic and Nuclear |
| Background: | | Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP 90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP 90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases. Studies have shown that murine HSP 90 exists as two forms, HSP 84 and HSP 86, coded by related but separate genes, with 86% amino acid sequence conservation. These forms are analogous to the two forms of human HSP 90, HSP 89 alpha and HSP 89 beta. In an unstressed mouse fibroblast, the basal level of HSP 84 is found to be double that of HSP 86. However, after heat shock, HSP 86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP 86, but not HSP 84, decreases. HSP 84 and HSP 86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| ListPrice: | | 285 |
| AppSummary: | | IF, IP, WB, IHC-Fr |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-HSP 90 BETA antibody, anti-HSP90 BETA antibody, anti-D6S182 antibody, anti-FLJ26984 antibody, anti-heat shock 90kD protein 1, beta antibody, anti-heat shock 90kDa protein 1, beta antibody, anti-heat shock 90kDa protein beta antibody, anti-heat shock protein 90kDa alpha (cytosolic), class B member 1 antibody, anti-heat shock protein beta antibody, anti-heat shock protein HSP 90 beta antibody, anti-HSP 84 antibody, anti-hsp 90 ab1 antibody, anti-HSP 90 antibody, anti-hsp 90ab1 antibody, anti-HSP84 antibody, anti-HSP90 antibody, anti-hsp90ab1 antibody, anti-HSP90B antibody, anti-HSP90BETA antibody, anti-HSPC2 antibody, anti-HSPCB antibody, anti-OTTHUMP00000039869 antibody |
| ProteinTarget: | | Hsp 84 |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | This product has been used in:Perdew GH et al. Localization and characterization of the 86- and 84-kDa heat shock proteins in Hepa 1c1c7 cells. Exp Cell Res 209:350-6 (1993). General / background references:Chen HS & Perdew GH Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex. J Biol Chem 269:27554-8 (1994). Moore SK et al. Characterization of the mouse 84-kD heat shock protein gene family. DNA Cell Biol 9:387-400 (1990). Moore SK et al. Murine 86- and 84-kDa heat shock proteins, cDNA sequences, chromosome assignments, and evolutionary origins. J Biol Chem 264:5343-51 (1989). |
| more information: | | company product webpage |
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