| CatalogCode: | NB120-2913 |
| ProductName: | Alpha COP Antibody |
| Product Description: | Rabbit Polyclonal anti-Alpha COP |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: MLTKFETKSARVKGLSFHP-C, corresponding to amino acids 1-19 of Rat alpha COP I. |
| Specificity: | Detects coatomer-protein I alpha (COP I alpha). |
| CrossReactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Immunocytochemistry: use at 3 ug/ml. Western Blot: use at 1 ug/ml. By Western blot, this antibody detects an ~140 kDa protein representing COPI alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha COP I using ab2913 results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. |
| Localization: | Cytoplasmic and Golgi Apparatus |
| Background: | Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | WB, ICC, ICC |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-Alpha coat protein antibody, anti-Alpha COP antibody, anti-Alpha COPI antibody, anti-AlphaCOP antibody, anti-Coatomer protein complex subunit alpha antibody, anti-Coatomer subunit alpha antibody, anti-COP A antibody, anti-COP I alpha antibody, anti-COPA antibody, anti-COPI alpha antibody, anti-FLJ26320 antibody, anti-HEP COP antibody, anti-HEPCOP antibody, anti-Xenin antibody |
| ProteinTarget: | Alpha COP |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Futatsumori M et al. Identification and characterization of novel isoforms of COP I subunits. J Biochem (Tokyo) 128:793-801 (2000). Shimoni Y et al. Lst1p and Sec24p cooperate in sorting of the plasma membrane ATPase into COPII vesicles in Saccharomyces cerevisiae. J Cell Biol 151:973-84 (2000). Barlowe C Traffic COPs of the early secretory pathway. Traffic 1:371-7 (2000). |
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