| CatalogCode: | NB120-2908 |
| ProductName: | Calreticulin Antibody |
| Product Description: | Chicken Polyclonal anti-Calreticulin |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: DEKEEDEEESPGQAKD, corresponding to amino acids 399-414 of Mouse Calreticulin - ER Marker. |
| CrossReactivity: | Cross-reacts with Human, Rabbit, Rat, Mouse, Dog and Hamster.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Chicken IgG. |
| Uses: | Western blot: use at a dilution of 1/2000.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract. |
| Localization: | Endoplasmic reticulum and secreted. Also found on the cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. |
| Background: | Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host_Name: | Chicken |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | IF, IP, WB, ICC |
| SpeciesSummary: | Ca, Ha, Hu, Mu, Rb, Rt |
| ALTnames: | anti-Autoantigen RO antibody, anti-CALR antibody, anti-CALR protein antibody, anti-Calregulin antibody, anti-Calreticulin antibody, anti-cC1qR antibody, anti-CRP55 antibody, anti-CRTC antibody, anti-ERp60 antibody, anti-FLJ26680 antibody, anti-grp60 antibody, anti-HACBP antibody, anti-RO antibody, anti-Sicca syndrome antigen A antibody, anti-SSA antibody |
| ProteinTarget: | Calreticulin |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Otteken A & Moss B Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin. J Biol Chem 271:97-103 (1996). Camacho P & Lechleiter JD Calreticulin inhibits repetitive intracellular Ca2+ waves. Cell 82:765-71 (1995). Wada I et al. Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms. J Biol Chem 270:20298-304 (1995). |
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