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| Catalog Code: | NB120-2906 |
| Product Name: | Beta Epithelial Sodium Channel Antibody |
| Product Description: | Rabbit Polyclonal anti-Beta Epithelial Sodium Channel |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Peptide sequence: N(619)YDSLRLQPLDVIESDSEGDAI(640). Immunizing peptide corresponds to amino acid residues 619-640 from human beta-ENaC. This sequence is 90% and 81% conserved in the rabbit, rat and mouse beta-ENaC, respectively. |
| Specificity: | Detects recombinant rat beta-epithelial sodium channel (beta-ENaC). |
| Cross Reactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | Immunoprecipitation: Assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user. By immunoprecipitation this antibody specifically binds to unglycosylated, ~75 kDa recombinant rat beta-ENaC protein. This product fails to detect beta-ENaC protein in Western blot procedures. |
| Background: | Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel's syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunoprecipitation |
| Species Summary: | Hu, Rt |
| Package Size: | 0.1 mg |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | General / background references:Ishibashi K & Marumo F Molecular cloning of a DEG/ENaC sodium channel cDNA from human testis. Biochem Biophys Res Commun 245:589-93 (1998). Barbry P & Hofman P Molecular biology of Na+ absorption. Am J Physiol 273:G571-85 (1997). |
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