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| Catalog Code: | NB120-2899 |
| Product Name: | Beta COP Antibody |
| Product Description: | Rabbit Polyclonal anti-Beta COP |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: E(496)AGELKPEEEITVGPVQK(513). Immunizing peptide corresponds to amino acid residues 496 - 513 of rat beta-COP. This sequence is completely conserved in human beta-COP. |
| Cross Reactivity: | Cross-reacts with Rat and Mouse.Expected to cross-react with Human due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml IgG purified Rabbit IgG. |
| Uses: | Immunofluorescence: use at a dilution of 1/2000.Western blot: use at duilution of 1/1000.Optimal dilutions / concentrations are best determined by the end user. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate.Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion. |
| Localization: | The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. |
| Background: | Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Mu, Rt |
| Alternate Names: | anti-Beta-coat protein antibody, anti-betacop antibody, anti-Coatomer beta subunit antibody, anti-Coatomer protein complex subunit beta 1 antibody, anti-Coatomer protein complex subunit beta antibody, anti-Coatomer subunit beta antibody, anti-COPB antibody, anti-COPB1 antibody, anti-DKFZp761K102 antibody, anti-FLJ10341 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 1 mg/ml BSA, 0.05% sodium azide |
| General References: | General / background references:Orci L et al. Bidirectional transport by distinct populations of COPI-coated vesicles. Cell 90:335-49 (1997). Schekman R & Orci L Coat proteins and vesicle budding. Science 271:1526-33 (1996). Lowe M & Kreis TE In vitro assembly and disassembly of coatomer. J Biol Chem 270:31364-71 (1995). Duden R et al. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 64:649-65 (1991). |
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