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| Catalog Code: | NB120-2849 |
| Product Name: | Dnmt1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Dnmt1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: AKGPAKRKPQEESE, corresponding to amino acids 171-185 of Human Dnmt 1. |
| Specificity: | Detects DNA methyltransferase 1 (Dmnt1). |
| Cross Reactivity: | Cross-reacts with Human.Does not cross-react with Mouse.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | In Western Blot this antibody detects a band of approximately 180 kDa (predicted molecular weight: 183 kDa). A doublet between 40 and 45 kDa is also seen on Western blots using this antibody. Can be blocked with <ab4920>Dnmt 1 peptide</ab>.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintenance and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1 mg/ml BSA |
| Application Summary: | Western Blot 0.5 - 1 ug/ml |
| Species Summary: | Hu, Mu(-), , |
| Alternate Names: | anti-DNMT-1 antibody, anti-DNMT 1 antibody, anti-DNMT antibody, anti-DNA 9cytosine-5-)-methylatransferase 1 antibody, anti-MCMT antibody, anti-DNA MTase Mmu1 antibody, anti-DNA methyltransferase M.HsaI antibody |
| Package Size: | 0.1 mg |
| Preservative: | Sodium Azide |
| General References: | General / background references:Araujo FD et al. The DNMT1 target recognition domain resides in the N terminus. J Biol Chem 276:6930-6 (2001). Aguirre-Arteta AM et al. Expression of an alternative Dnmt1 isoform during muscle differentiation. Cell Growth Differ 11:551-9 (2000). |
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