| CatalogCode: | | NB120-2827 |
| ProductName: | | Ryanodine Receptor Antibody |
| Product Description: | | Mouse Monoclonal anti-Ryanodine Receptor (C3-33) |
| Clone: | | C3-33 |
| Clonality: | | Monoclonal |
| Immunogen: | | Canine cardiac ryanodine receptor |
| Specificity: | | Detects ryanodine receptor 2 (RyR 2) and weakly detects RyR 1. |
| CrossReactivity: | | Cross-reacts with Rabbit, Rat, Dog, Guinea pig, Chicken, Fish and Amphibians.Not yet tested in other species. |
| Packaging: | | 0.1 mg purified Mouse ascites. |
| Uses: | | Immunohistochemistry: use at a concentration of 1 ug/ml. Immunoprecipitation: Assay dependent. Western Blot:use at a concentration of 1 ug/ml. FACS: Assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization. |
| Localization: | | Membrane; Multi-pass membrane protein |
| Background: | | The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | PBS |
| ListPrice: | | 285 |
| AppSummary: | | IF, IP, WB, ICC, IHC-Fr |
| SpeciesSummary: | | Rb, Rt, Ca, GP, Ch, Fi, Am |
| ALTnames: | | anti-ARVC 2 antibody, anti-ARVC2 antibody, anti-ARVD 2 antibody, anti-ARVD2 antibody, anti-Brain ryanodine receptor calcium release channel antibody, anti-Brain type ryanodine receptor antibody, anti-Cardiac muscle ryanodine receptor antibody, anti-Cardiac muscle ryanodine receptor calcium release channel antibody, anti-Cardiac muscle type ryanodine receptor antibody, anti-CCO antibody, anti-Central core disease of muscle antibody, anti-HBRR antibody, anti-hRYR 2 antibody, anti-MHS antibody, anti-MHS1 antibody, anti-Ryanodine receptor 1 antibody, anti-Ryanodine receptor 2 antibody, anti-Ryanodine receptor 3 antibody, anti-Ryanodine receptor type1 antibody, anti-RYDR antibody, anti-RYR 1 antibody, anti-RYR 2 antibody, anti-RYR 3 antibody, anti-RYR antibody, anti-RYR1 antibody, anti-RYR2 antibody, anti-RYR3 antibody, anti-Sarcoplasmic reticulum calcium release channel antibody, anti-Skeletal muscle calcium release channel antibody, anti-Skeletal muscle ryanodine receptor antibody, anti-Skeletal muscle type ryanodine receptor antibody, anti-SKRR antibody, anti-Type 1 like ryanodine receptor antibody, anti-VTSIP antibody |
| ProteinTarget: | | Ryanodine Receptor |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | This product has been used in:Du GG et al. Functional characterization of mutants in the predicted pore region of the rabbit cardiac muscle Ca(2+) release channel (ryanodine receptor isoform 2). J Biol Chem 276:31760-71 (2001). Yamada KA et al. Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum. J Cardiovasc Pharmacol 36:14-21 (2000). Lai FA et al. Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle. Am J Physiol 263:C365-72 (1992). General / background references:Moore RA et al. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol 140:843-51 (1998). Grunwald R & Meissner G Lumenal sites and C terminus accessibility of the skeletal muscle calcium release channel (ryanodine receptor). J Biol Chem 270:11338-47 (1995). Carl SL et al. Immunolocalization of sarcolemmal dihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium. J Cell Biol 129:672-82 (1995). Junker J et al. Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors. J Biol Chem 269:1627-34 (1994). Xu L et al. Evidence for a Ca(2+)-gated ryanodine-sensitive Ca2+ release channel in visceral smooth muscle. Proc Natl Acad Sci U S A 91:3294-8 (1994). McPherson PS & Campbell KP The ryanodine receptor/Ca2+ release channel. J Biol Chem 268:13765-8 (1993). |
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