| CatalogCode: | | NB120-2805 |
| ProductName: | | Muscarinic 2 Acetylcholine Receptor Antibody |
| Product Description: | | Mouse Monoclonal anti-Muscarinic 2 Acetylcholine Receptor (31-1D1) |
| Clone: | | 31-1D1 |
| Clonality: | | Monoclonal |
| Immunogen: | | Purified porcine cardiac m2 muscarinic acetylcholine receptor. |
| Specificity: | | This antibody is specific for the m2 mAChR subtype. |
| CrossReactivity: | | Cross-reacts with Human, Rat, Mouse and Pig.Does not cross-react with Chicken.Not yet tested in other species. |
| Packaging: | | 0.125 ml purified Mouse ascites. |
| Uses: | | Western Blot: use at a diltuion of 1:1000.Immunoprecipitation: assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects 60-80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR from pig heart. |
| Localization: | | Plasma membrane |
| Background: | | Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | PBS |
| ListPrice: | | 285 |
| AppSummary: | | IP, WB |
| SpeciesSummary: | | Hu, Rt, Mu, Po, Ch (-) |
| ALTnames: | | anti-7TM receptor antibody, anti-Cholinergic receptor muscarinic 2 antibody, anti-Cholinergic receptor, muscarinic 2, isoform a antibody, anti-CHRM 2 antibody, anti-CHRM2 antibody, anti-FLJ43243 antibody, anti-HM 2 antibody, anti-HM2 antibody, anti-MGC120006 antibody, anti-MGC120007 antibody, anti-Muscarinic acetylcholine receptor M2 antibody, anti-Muscarinic M2 receptor antibody |
| ProteinTarget: | | Muscarinic 2 Acetylcholine Receptor |
| PackageSize: | | 0.125 ml |
| GeneralRef: | | This product has been used in:Luetje CW et al. Isolation and characterization of monoclonal antibodies specific for the cardiac muscarinic acetylcholine receptor. Biochemistry 26:6892-6 (1987). General / background references:Wreggett KA & Wells JW Cooperativity manifest in the binding properties of purified cardiac muscarinic receptors. J Biol Chem 270:22488-99 (1995). Wang P et al. Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins. J Pharmacol Exp Ther 273:959-66 (1995). |
| more information: | | company product webpage |
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