| CatalogCode: | NB120-2804 |
| ProductName: | Nicotinic Acetylcholine Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Nicotinic Acetylcholine Receptor (88B) |
| Clone: | 88B |
| Clonality: | Monoclonal |
| Immunogen: | Purified Torpedo californica acetylcholine receptor. |
| Specificity: | Detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit. This antibody does not detect the alpha 1 and beta 1 subunits. |
| CrossReactivity: | Cross-reacts with Rat, Mouse, Chicken, Fish and Amphibians.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | Immunohistochemistry: use at a dilution of 1/1000. Western Blot: use at a dilution of 1/5000 Immunoprecipitation: Assay dependent.Not tested in other applications. Optimal dilutions should be determined by the end user.By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates.Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle results in strong staining of the motor endplate. Cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor. |
| Background: | Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1?s, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | IP, WB, IHC-Fr |
| SpeciesSummary: | Rt, Mu, Ch, Fi, Am |
| ALTnames: | anti-Acetylcholine receptor subunit alpha antibody, anti-ACHRA antibody, anti-ACHRD antibody, anti-Cholinergic receptor nicotinic alpha 1 antibody, anti-Cholinergic receptor nicotinic alpha 1 muscle antibody, anti-Cholinergic receptor nicotinic alpha polypeptide 1 antibody, anti-Cholinergic receptor nicotinic alpha polypeptide 1 muscle antibody, anti-CHRNA 1 antibody, anti-CHRNA antibody, anti-CHRNA1 antibody, anti-CMS 2A antibody, anti-CMS2A antibody, anti-FCCMS antibody, anti-Nicotinic acetylcholine receptor alpha subunit antibody, anti-SCCMS antibody |
| ProteinTarget: | Nicotinic Acetylcholine Receptor |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Froehner SC et al. Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor. J Biol Chem 258:7112-20 (1983). General / background references:Xie MH et al. Direct demonstration of MuSK involvement in acetylcholine receptor clustering through identification of agonist ScFv. Nat Biotechnol 15:768-71 (1997). Bloch RJ et al. Association of acetylcholine receptors with peripheral membrane proteins: evidence from antibody-induced coaggregation. J Membr Biol 138:13-28 (1994). Karlin A Structure of nicotinic acetylcholine receptors. Curr Opin Neurobiol 3:299-309 (1993). |
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