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| Catalog Code: | NB120-2788 |
| Product Name: | Hsc70 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsc70 (13D3) |
| Clone: | 13D3 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Mouse spermatogenic cell protein. |
| Specificity: | This antibody displays slight cross-reactivity to HSP 70. |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.05 ml Mouse ascites. |
| Uses: | Immunohistochemistry: use at a dilution of 1/100. Western Blot: use at a dilution of 1/5000.Immunoprecipitation: Assay dependent. Not tested in any other applications.Optimal dilutions should be determined by the end user.By Western blot, this antibody detects a 70 kDa protein representing Hsc 70. By 2D gel electrophoresis, this antibody binds strongly to Hsc 70 and faintly to HSP 70 both before and after heat shock. Immunohistochemical staining of Hsc 70 in mouse spermatids/spermatozoa results in staining restricted to the post acrosomal region in condensing spermatids and to the midpiece in spermatozoa. |
| Background: | The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP 78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsc 75, a 75 kDa protein that is found within the mitochondria. Hsc 70 (also known as Hsc 71, Hsc 73, Hsc 73, p72, prp 73) is expressed constitutively and is slightly heat-inducible. Hsc 70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. Hsc 70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgM |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry-Fr, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-HSC70 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| General References: | This product has been used in:Maekawa M et al. Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells. Biol Reprod 40:843-52 (1989). General / background references:Leung SM & Hightower LE A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family. J Biol Chem 272:2607-14 (1997). Terlecky SR et al. Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein. J Biol Chem 267:9202-9 (1992). Gething MJ & Sambrook J Protein folding in the cell. Nature 355:33-45 (1992). Allen RL et al. A novel hsp70-like protein (P70) is present in mouse spermatogenic cells. Mol Cell Biol 8:828-32 (1988). |
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