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| Catalog Code: | NB120-2781 |
| Product Name: | NCoR2 - CHIP Grade Antibody |
| Product Description: | Mouse Monoclonal anti-NCoR2 - CHIP Grade (1542) |
| Clone: | 1542 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: CGGA-SASDPHREKTQ, corresponding to amino acids 994-1005 of Human NCoR2. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | ChIP: Use 3ul for 10<sup>6</sup> cells.IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 4 ug/ml. Detects a band of approximately 250 and 220 kDa (predicted molecular weight: 274 kDa). Can be blocked with <ab4910>Retinoic Acid Receptor alpha peptide</ab> |
| Background: | Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing Mediator of Retinoic acid & Thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS, 1 mg/ml BSA, 0.05% sodium azide |
| Application Summary: | chromatin immunoprecipitation, immunohistochemistry-Fr, Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-NCoR 2 antibody, anti-nuclear receptor co-repressor 2 antibody, anti-Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody, anti-SMRT antibody, anti-SMRTE antibody |
| Package Size: | 0.1 mg |
| Notes Main: | In ChIP NB120-2781 gives a maximum at 60 min (more than 60%) whereas NB120-2781 gives a minimum (around 5%) at this time and NB120-2780 gives a minimum (less than 5%) at 30 min whereas NB120-2781 gives a maximum (50%). This protein is corepressor of the nuclear hormone receptors and is not supposed to be recruited in presence of the hormone (here oestrogen), and should be maximal before induction, which is not the case here for both antibodies! |
| General References: | General / background references:Hudelist G et al. Expression of sex steroid receptors and their co-factors in normal and malignant breast tissue: AIB1 is a carcinoma-specific co-activator. Breast Cancer Res Treat 78:193-204 (2003). Thenot S et al. Estrogen receptor cofactors expression in breast and endometrial human cancer cells. Mol Cell Endocrinol 156:85-93 (1999). |
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