| CatalogCode: | NB120-2775 |
| ProductName: | Rhodopsin Kinase 1a Antibody |
| Product Description: | Mouse Monoclonal anti-Rhodopsin Kinase 1a (G8) |
| Clone: | G8 |
| Clonality: | Monoclonal |
| Immunogen: | Full length human GRK1 |
| Epitope: | This antibody recognizes an epitope in the C-terminal region of GRK1a. |
| Specificity: | Detects G-protein-associated rhodopsin kinase 1a (GRK1a). |
| CrossReactivity: | Cross-reacts with Human, Rat, Mouse, Cow and Chicken.Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse IgG. |
| Uses: | Immunofluorescence: use at 1 ug/ml Western Blot: use at 1 ug/ml By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with ab2775 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. |
| Background: | The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| AppSummary: | IF, IP, WB |
| SpeciesSummary: | Bv, Ch, Hu, Mu, Rt |
| ALTnames: | anti-G protein-coupled receptor kinase 1 antibody, anti-Rhodopsin kinase antibody, anti-RHOK antibody, anti-RK antibody |
| ProteinTarget: | Rhodopsin Kinase 1a |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Bensaoula T et al. Histopathologic and immunocytochemical analysis of the retina and ocular tissues in Batten disease. Ophthalmology 107:1746-53 (2000). Zhao X et al. A novel form of rhodopsin kinase from chicken retina and pineal gland. FEBS Lett 454:115-21 (1999). |
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