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| Catalog Code: | NB120-2765 |
| Product Name: | Progesterone Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Progesterone Receptor (Alpha PR6) |
| Clone: | Alpha PR6 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Progesterone receptor (PR) purified from chick oviduct cytosol. |
| Specificity: | Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor. |
| Cross Reactivity: | Cross-reacts with Human, Rabbit, Rat, Mouse, Cow, Guinea pig, Sheep and Chicken. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | Immunohistochemistry: use at a dilution of 20 ug/ml. Western Blot: use at a dilution of 1 ug/ml. Immunocytochemistry: Assay dependent. Immunoprecipitation: Assay dependent.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a 110 kDa protein representing the B form of the PR in chickens and rabbits as well a 120 kDa doublet or triplet bands in humans. |
| Localization: | Nuclear |
| Background: | The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry (F): 20 ug/ml: IHC (P): 5 ug/ml, immunoprecipitation Use at an assay dependent dilution, Western Blot 1 ug/ml, Immunocytochemistry Use at an assay dependent dilution |
| Species Summary: | Bv, Ch, Hu, Mu, Rt, Rb, Gp, Sh, |
| Alternate Names: | anti-NR3C3 antibody, anti-PGR antibody, anti-PR antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide |
| General References: | This product has been used in:Joensuu TK et al. Progesterone-induced avidin as a marker of cytodifferentiation in the oviduct: comparison to ovalbumin. Endocrinology 126:1143-55 (1990). Pekki A et al. Progesterone receptor concentration differences in the chick oviduct cells and apparent down-regulation by ligand. A semiquantitative immunohistochemical study. J Steroid Biochem 34:351-4 (1989). Bergeron C et al. Immunocytochemical study of progesterone receptors in the human endometrium during the menstrual cycle. Lab Invest 59:862-9 (1988). Schneider W et al. Interaction of murine progesterone receptors with specific monoclonal antibodies to the avian progesterone receptor. J Steroid Biochem 29:297-306 (1988). Bergeron C et al. Immunocytochemical study of progesterone receptors in hyperplastic and neoplastic endometrial tissues. Cancer Res 48:6132-6 (1988). Estes PA et al. Immunologic analysis of human breast cancer progesterone receptors. 1. Immunoaffinity purification of transformed receptors and production of monoclonal antibodies. Biochemistry 26:6250-62 (1987). Sullivan WP et al. Preparation of monoclonal antibodies to the avian progesterone receptor. Endocrinology 119:1549-57 (1986). General / background references:Yoshimura Y & Bahr JM Localization of progesterone receptors in pre- and postovulatory follicles of the domestic hen. Endocrinology 128:323-30 (1991). |
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