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| Catalog Code: | NB120-2738 |
| Product Name: | LAP2 Antibody |
| Product Description: | Hamster Monoclonal anti-LAP2 (RL29) |
| Clone: | RL29 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Pore complex-lamina fraction isolated from rat liver nuclear envelopes. |
| Specificity: | Detects Lamin Associated Polypeptide (LAP) 2 from various rat tissues but does not cross-react with LAP 1A, 1B & 1C.This antibody recognizes a single 53 kDa protein representing LAP 2 in rat liver nuclear envelope fractions. |
| Cross Reactivity: | Cross-reacts with Rat.Not yet tested in other species. |
| Packaging: | 0.2 ml IgG purified Hamster IgG. |
| Uses: | Immunofluorescence: use at 1:100 Immunoprecipitation: Assay dependentWestern Blot: use at 1:1000Optimal dilutions should be determined by the individual. |
| Localization: | Nuclear and Secreted |
| Background: | In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Hamster |
| Buffer: | PBS |
| Application Summary: | immunofluorescence 1:100, immunoprecipitation , Western Blot 1:1,000 |
| Species Summary: | Rt |
| Alternate Names: | anti-CMD 1T antibody, anti-CMD1T antibody,anti-Lamina associated polypeptide 2 antibody , anti-Lamina associated polypeptide 2 isoform alpha antibody, anti-Lamina associated polypeptide 2 isoforms beta/gamma antibody, anti-LAP 2 antibody, anti-MGC61508 antibody, anti-PRO0868 antibody, anti-thymopoietin antibody, anti-Thymopoietin isoform alpha antibody, anti-Thymopoietin isoforms beta/gamma antibody, anti-Thymopoietin related peptide isoform alpha antibody, anti-Thymopoietin related peptide isoforms beta/gamma antibody, anti-TMPO antibody, anti-TP alpha antibody, anti-TP antibody, anti-TP beta/gamma antibody, anti-TPRP isoform alpha antibody, anti-TPRP isoforms beta/gamma antibody |
| Package Size: | 0.2 ml |
| Preservative: | 0.05% sodium azide |
| General References: | This product has been used in:Foisner R & Gerace L Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73:1267-79 (1993). General / background references:Yang L et al. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol 139:1077-87 (1997). Senior A & Gerace L Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina. J Cell Biol 107:2029-36 (1988). |
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