| request information: |
|
| Catalog Code: | NB120-2737 |
| Product Name: | LAP1 Antibody |
| Product Description: | Mouse Monoclonal anti-LAP1 (RL13) |
| Clone: | RL13 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Pore complex-lamina fraction isolated from rat liver nuclear envelopes. |
| Specificity: | Detects lamin associated polypeptide (LAP) 1A, 1B & 1C from rat tissues. This antibody does not cross-react with LAP 2.This antibody detects 3 distinct proteins of molecular weights 75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. |
| Cross Reactivity: | Cross-reacts with Rat.Not yet tested in other species. |
| Packaging: | 0.2 ml IgG purified Mouse IgG. |
| Uses: | Immunofluorescence 1:100 Immunoprecipitation: Assay dependent Western Blot 1:200 Optimal dilutions should be determined by the individual. |
| Localization: | Immunofluorescence staining of LAP 1 in rat liver with this antibody results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining. |
| Background: | In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others.LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only.In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunofluorescence 1:100 , immunoprecipitation , Western Blot 1:200 |
| Species Summary: | Rt |
| Alternate Names: | anti-Densin 180 antibody, anti-DKFZp686I1147 antibody, anti-KIAA1365 antibody, anti-Lamin associated polypeptide 1 antibody, anti-LAP 1 antibody, anti-Leucine rich repeat containing 7 antibody, anti-Leucine rich repeat containing protein 7 antibody, anti-Leucine-rich repeat-containing protein 7 antibody, anti-Lrrc 7 antibody, anti-Lrrc7 antibody, anti-MGC144918 antibody, anti-OTTHUMP00000065336 antibody, anti-OTTHUMP00000065337 antibody, anti-OTTHUMP00000065338 antibody, anti-Protein Lap 1 antibody, anti-Protein Lap1 antibody |
| Package Size: | 0.2 ml |
| Preservative: | 0.05% sodium azide |
| General References: | This product has been used in:Yang L et al. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol 139:1077-87 (1997). Guan T et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). Martin L et al. cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane. J Biol Chem 270:8822-8 (1995). Foisner R & Gerace L Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation. Cell 73:1267-79 (1993). Senior A & Gerace L Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina. J Cell Biol 107:2029-36 (1988). |
order or
more information: | company product webpage |