| CatalogCode: | NB120-26824 |
| ProductName: | EpoR Antibody |
| Product Description: | Sheep Polyclonal anti-EpoR |
| Clonality: | Polyclonal |
| Immunogen: | The immunogen used was the cDNA of the extracellular domain of human EpoR expressed as a glutathione-S-transferase (GST) fusion protein in E. coli. |
| Specificity: | Antibody titers and specificity were analyzed by ELISA assay using control samples by varying concentration of GST-EpoR and control anti-GST antibody. |
| CrossReactivity: | This product is reactive against human. It has not been tested in other species. |
| Packaging: | 0.25 mg protein G purified Sheep antisera. |
| Uses: | Affinity purified antibody was found to detect EpoR at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. The Western blot concentration used was 10 ug/ml. This antibody will recognize EpoR-GST at its migration of 49 kD on SDS-PAGE. High quality secondary anti-sheep reagents are essential for this assay. Past experiments recommend the use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents. |
| Background: | The human erythropoietin receptor (EpoR) is located on stem and mature cells of the hematopoietic system. When activated by its native ligand, erythropoietin (Epo), EpoR mediates proliferation and maturation of the erythroid precursor cells leading to expansion of red cell populations. Human recombinant Epo is currently used for treatment of patients undergoing erythrocyte depletion due to dialysis for kidney failure. |
| ProductRef: | Nakatsuji T. Long-term survival of a piece of rat bone marrow graft with the bone in syngeneic rat spleen: new roles of bone marrow mast cells and host spleen megakaryocytes secreting vascular endothelial growth factor (VEGF). Haema 2005; 8(3):450-457 |
| Storage: | Fairchild Benyo D, Conrad KP. Expression of the erythropoietin receptor by trophoblast cellsin the human placenta. Biol Reprod. 1999 Apr;60(4):861-70 Harris KW, Mitchell RA, Winkelmann JC. Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli. J Biol Chem. 1992 Jul 25;267(21):15205-9 |
| Purity: | Store at 4 degrees short term and -20 degrees C long term. |
| Isotype: | protein G purified |
| Buffer: | Sheep |
| ListPrice: | 0.15 M PBS pH 7.4 |
| NovusRef: | 325 |
| SpeciesSummary: | ELISA, IP, WB, ICC |
| ALTnames: | Hu |
| ProteinTarget: | anti-mgc138358 antibody, anti-erythropoietin receptor antibody |
| Homology: | 0.25 mg |