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| Catalog Code: | NB120-2562 |
| Product Name: | MSK 1 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-MSK 1 [Thr581] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Available on request. |
| Specificity: | This antibody detects 90 kDa MSK 1 only when phosphorylated at Thr581. It does not cross-react with non-phosphorylated MSK 1, or MSK 1 phosphorylated at other sites. It does not cross-react with RSK 1, RSK 2 or RSK 3. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 1 - 2 ug/ml. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear; predominantly. Cytoplasmic; partially. |
| Background: | MSK 1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK 1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK 1 at Ser 364, Ser381 and Thr574 is critical for RSK 1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK 1 and maybe important for MSK 1 activity as well. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS pH7.2 with 50% glycerol, 1% BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-90 kDa Ribosomal Protein S6 Kinase 5 antibody, anti-MSPK1 antibody, anti-Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody, anti-Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody, anti-Ribosomal Protein S6 Kinase Alpha 5 antibody, anti-RLPK antibody, anti-RPS6KA5 antibody, anti-RSK Like Protein Kinase antibody, anti-RSKL antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.02% sodium azide |
| Phosphorylated: | Thr581 |
| General References: | This product has been used in:Deak M et al. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J 17:4426-41 (1998). Pierrat B et al. RSK-B, a novel ribosomal S6 kinase family member, is a CREB kinase under dominant control of p38alpha mitogen-activated protein kinase (p38alphaMAPK). J Biol Chem 273:29661-71 (1998). |
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more information: | company product webpage |