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| Catalog Code: | NB120-2365 |
| Product Name: | Catenin beta Antibody |
| Product Description: | Rabbit Polyclonal anti-Catenin beta |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody reacts with a 92kDa protein, known as beta Catenin. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse, Chicken and Xenopus laevis. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Rabbit antisera. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:100-1:200 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Background: | The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot, |
| Species Summary: | Ch, Hu, Mu, Rt, Xp, |
| Alternate Names: | anti-Beta catenin antibody, anti-Cadherin associated protein antibody, anti-Catenin beta 1 antibody, anti-CATNB antibody, anti-CTNNB antibody, anti-CTNNB1 antibody |
| Package Size: | 0.5 ml |
| Preservative: | sodium azide |
| General References: | General / background references:Koslov ER et al. Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin. J Biol Chem 272:27301-6 (1997). Hazan RB et al. Vinculin is associated with the E-cadherin adhesion complex. J Biol Chem 272:32448-53 (1997). Rimm DL et al. Reduced alpha-catenin and E-cadherin expression in breast cancer. Lab Invest 72:506-12 (1995). |
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