| request information: |
|
| Catalog Code: | NB120-2364 |
| Product Name: | Catenin (alpha) Antibody |
| Product Description: | Rabbit Polyclonal anti-Catenin (alpha) |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody reacts with a 102kDa protein, known as alpha Catenin. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Xenopus laevis. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Rabbit antisera. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Cytoplasmic, cell membrane |
| Background: | The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kDa) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot, |
| Species Summary: | Hu, Mu, Xp, |
| Alternate Names: | anti-alpha catenin antibody, anti-Alpha E Catenin antibody, anti-alphaE catenin antibody, anti-Cadherin associated protein 102kDa antibody, anti-Cadherin associated protein antibody, anti-CAP 102 antibody, anti-CAP102 antibody, anti-Catenin (cadherin associated protein) alpha 1 102kDa antibody, anti-Catenin alpha 1 antibody, anti-CTNNA 1 antibody, anti-CTNNA1 antibody, anti-FLJ36832 antibody, anti-NY REN 13 antigen antibody |
| Package Size: | 0.5 ml |
| Preservative: | sodium azide |
| General References: | General / background references:Koslov ER et al. Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin. J Biol Chem 272:27301-6 (1997). Hazan RB et al. Vinculin is associated with the E-cadherin adhesion complex. J Biol Chem 272:32448-53 (1997). Rimm DL et al. Reduced alpha-catenin and E-cadherin expression in breast cancer. Lab Invest 72:506-12 (1995). |
order or
more information: | company product webpage |