| CatalogCode: | NB120-2357 |
| ProductName: | Cdc25A Antibody |
| Product Description: | Mouse Monoclonal anti-Cdc25A (DCS-120 + DCS-121) |
| Clone: | DCS-120 + DCS-121 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | This antibody reacts with a 65 kD protein known as Cdc25A. |
| CrossReactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Mouse ascites. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Nuclear membrane. |
| Control: | Tonsil. |
| Background: | Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | PBS |
| ListPrice: | 325 |
| AppSummary: | IHC-Fr, IHC-P, IP, WB |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-Cell division cycle 25A isoform a antibody, anti-Cell division cycle 25A isoform b antibody, anti-Dual specificity phosphatase CDC25A antibody, anti-M phase inducer phosphatase 1 antibody |
| ProteinTarget: | Cdc25A |
| PackageSize: | 0.5 ml |
| GeneralRef: | This product has been used in:Leisser C et al. Subcellular localisation of Cdc25A determines cell fate. Cell Death Differ 11:134 (2004). General / background references:Draetta et al J Biochem Biophys Acta 1332:53 (1997).Gabrielli et al Oncogene 15:749 (1997). |
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