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| Catalog Code: | NB120-2353 |
| Product Name: | MSH2 Antibody |
| Product Description: | Mouse Monoclonal anti-MSH2 (25D12) |
| Clone: | 25D12 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Immunogen: | Recombinant human MSH2 protein. |
| Specificity: | This antibody reacts with a 102 kD protein known as MSH2 (Mismatch Repair protein 2). |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml Mouse Tissue culture supernatant. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | Nuclear. |
| Background: | MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Buffer: | Tissue culture supernatant with sodium azide |
| Application Summary: | immunohistochemistry-Fr, immunohistochemistry-P |
| Species Summary: | Hu |
| Alternate Names: | anti-MSH2 antibody, anti-MSH-2 antibody, anti-mutS homolog 2 antibody, anti-COCA1 antibody, anti-Colon Cancer 1 antibody, anti-FCC1 antibody, anti-Cancer 1 antibody, anti-HNPCC1 antibody, anti-HNPCC antibody, anti-Hereditary Nonpolyposis Colon Cancer |
| Package Size: | 0.5 ml |
| General References: | General / background references:Maliaka YK et al. CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. Hum Genet 97:251-5 (1996). Palombo F et al. hMutSbeta, a heterodimer of hMSH2 and hMSH3, binds to insertion/deletion loops in DNA. Curr Biol 6:1181-4 (1996). Kolodner RD et al. Structure of the human MSH2 locus and analysis of two Muir-Torre kindreds for msh2 mutations. Genomics 24:516-26 (1994). Fishel R et al. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell 75:1027-38 (1993). |
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