| CatalogCode: | NB120-22244 |
| ProductName: | c-Jun Antibody |
| Product Description: | Rabbit Polyclonal anti-c-Jun [Ser63] |
| Clonality: | Polyclonal |
| Immunogen: | This antibody was developed against a synthetic phosphorylated peptide corresponding to amino acids 59-67 ([BLAST]DLLTS*PDVG[/BLAST]) of human c-Jun; Genbank accession no. NP_002219.1. This peptide sequence is reported by NCBI to be broady conserved. Specifically, the immunogen is 100% conserved between human, monkey, mouse rat, rabbit, pig, cow and sheep. Note: S* refers to phosphorylated serine. |
| Specificity: | Researchers are encouraged to optimize dilutions for their specific testing, and to use the sequence and accession number (NP_000475.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their s |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Rabbit, Cow, Monkey, Pig and Sheep. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Rabbit antisera. |
| Uses: | ELISA: 1/100 - 1/1000. |
| Localization: | Nuclear |
| Background: | . c-Jun is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. C-Jun forms a complex with c-Fos, forming the transcription factor AP-1 (reviewed in 1). AP-1 is a generic term for a dimeric transcription factor that contains members from the Jun, Fos, ATF, and MAF protein families. AP-1 complexes recognize the same sequence motif, termed TRE (TPA responsive element), which is also referred to as the AP-1 binding site. AP-1 activity can be regulated by dimer composition, transcription, post-translational modification and interactions with other proteins. AP-1 proteins play key roles in cell proliferation, differentiation, apoptosis, angiogenesis and tumour invasion. The c-Jun gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | ELISA |
| SpeciesSummary: | Bv, Hu, Mu, Po, Mk, Rt, Rb, Sh |
| ProteinTarget: | c-Jun (phospho S63) |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Eferl R & Wagner EF AP-1: a double-edged sword in tumorigenesis. Nat Rev Cancer 3:859-68 (2003). |
| Phosphorylated: | Ser63 |
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