| request information: |
|
| Catalog Code: | NB120-22104 |
| Product Name: | SAE2 Antibody |
| Product Description: | Rabbit Polyclonal anti-SAE2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | A synthetic peptide within amino acid residues 160-210 of human SAE2 was used as immunogen. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Xenopus laevis, Zebrafish and Chicken. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Rabbit antisera. |
| Uses: | WB: Detects a band of approximately 71 kDa. |
| Localization: | Cytoplasmic |
| Background: | Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highl |
| Application Summary: | Western Blot 0.5 - 2 ug/ml |
| Species Summary: | Ch, Hu, Mu, Rt, Ze, Xp, |
| Alternate Names: | anti-UBA2 antibody, anti-SAE2 antibody, anti-SUMO-1 activating enzyme subunit 2 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide |
| General References: | General / background references:Tatham MH et al. Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nat Struct Mol Biol 12:67-74 (2005). Desterro JM et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem 274:10618-24 (1999). |
order or
more information: | company product webpage |