| CatalogCode: | NB120-22071 |
| ProductName: | IKB alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-IKB alpha |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide corresponding to amino acids 34-48 of human IkBa was used as the immunogen. It will cross-react with mouse and rat IkBa, based on BLAST. |
| Specificity: | In Jurkat, a 40 kDa band is observed. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (93% identity with immunogen) and Cow (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Rabbit antisera. |
| Uses: | WB: Use at a concentration of 1 - 3 ug/ml. Detects a band of approximately 40 kDa. |
| Localization: | Cytoplasmic |
| Control: | Jurkat |
| Background: | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first genes induced following NF-kB activation is IkBa. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Bv, Hu, Mu, Rt |
| ProteinTarget: | IKB alpha |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Yamaoka S et al. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation. Cell 93:1231-40 (1998). Verma IM & Stevenson J IkappaB kinase: beginning, not the end. Proc Natl Acad Sci U S A 94:11758-60 (1997). DiDonato JA et al. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 388:548-54 (1997). Régnier CH et al. Identification and characterization of an IkappaB kinase. Cell 90:373-83 (1997). Mercurio F et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science 278:860-6 (1997). Verma IM et al. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev 9:2723-35 (1995). |