| request information: |
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| Catalog Code: | NB120-21699 |
| Product Name: | Cyclin D1 Antibody |
| Product Description: | Rabbit Monoclonal anti-Cyclin D1 (SP4) |
| Clone: | SP4 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | A synthetic peptide from C-terminus of human cyclin D1. |
| Specificity: | Recognises Cyclin D1. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 7 ml Rabbit Tissue culture supernatant |
| Uses: | IHC-P: Use neat. Perform heat mediated antigen retrieval before commencing with IHC staining protocol; in particular: perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.Suggested primary antibody incubation period: 30 minutes at room temperature.IHC-Fr: Use neat. Note: samples are acetone fixed.Suggested primary antibody incubation period: 30 minutes at room temperature.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Whole serum |
| Application Summary: | immunohistochemistry-Fr, immunohistochemistry-P |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-B cell ccl/lymphoma 1 antibody, anti-B cell leukemia 1 antibody, anti-BCL1 antibody, anti-BCL1 oncogene antibody, anti-CCND 1 antibody, anti-CCND1 antibody, anti-CCND1 protein antibody, anti-CCND1/IGHG1 fusion gene antibody, anti-D11S287E antibody, anti-G1/S specific cyclin D1 antibody, anti-Parathyroid adenomatosis 1 antibody, anti-PRAD1 antibody, anti-PRAD1 oncogene antibody, anti-U21B31 antibody |
| Package Size: | 7 ml |
| Preservative: | none |
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more information: | company product webpage |
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