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| Catalog Code: | NB120-21303 |
| Product Name: | NADE Antibody |
| Product Description: | Rabbit Polyclonal anti-NADE |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | A 14 amino acid synthetic peptide from near the middle of NADE (Human). |
| Cross Reactivity: | Cross-reacts with Human and Mouse. |
| Packaging: | 0.05 mg ion exchange chromatography purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.5 - 2 ug/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 13 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nucleus. Cytoplasm. Note=Shuttles between the cytoplasm and the nucleus. Associates with replicating mitochondria (By similarity). |
| Background: | The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Coexpression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-BEX3 antibody, anti-Brain expressed X linked 3 (mouse) homolog antibody, anti-Nerve growth factor receptor (TNFRSF16) associated protein 1 antibody, anti-Nerve growth factor receptor associated protein 1 antibody, anti-NGFRAP1 antibody, anti-Ovarian granulosa cell 13.0 kDa protein HGR74 antibody, anti-Ovarian granulosa cell protein antibody, anti-p75NTR associated cell death executor antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.02% Sodium Azide |
| General References: | General / background references:Gentry JJ et al. The p75 neurotrophin receptor: multiple interactors and numerous functions. Prog Brain Res 146:25-39 (2004). Yoon K et al. Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis. Biochem Biophys Res Commun 319:649-54 (2004). Park JA et al. Co-induction of p75NTR and p75NTR-associated death executor in neurons after zinc exposure in cortical culture or transient ischemia in the rat. J Neurosci 20:9096-103 (2000). Mukai J et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J Biol Chem 275:17566-70 (2000). Rapp G et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol 9:479-85 (1990). |
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