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| Catalog Code: | NB120-1998 |
| Product Name: | AIF Antibody |
| Product Description: | Rabbit Polyclonal anti-AIF |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Peptide corresponding to amino acids 517 to531 of human AIF (2). This sequence is identical tothose of mouse and rat AIF (2). |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Rabbit antisera. |
| Uses: | Western blot gives a band at 67 kD. |
| Background: | Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 0.25 - 1 ug/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Apoptosis inducing factor antibody, anti-Harlequin antibody, anti-Hq antibody, anti-mAIF antibody, anti-MGC5706 antibody, anti-PDCD8 antibody, anti-Programmed cell death 8 isoform 1 antibody, anti-Programmed cell death 8 isoform 2 antibody, anti-Programmed cell death 8 isoform 3 antibody, anti-Programmed cell death protein 8 mitochondrial precursor antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% sodium azide |
| Notes Main: | Apoptosis Inducing Factor |
| General References: | General / background references:Daugas E et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J 14:729-39 (2000). Zamzami N & Kroemer G Condensed matter in cell death. Nature 401:127-8 (1999). Susin SA et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397:441-6 (1999). |
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more information: | company product webpage |