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| Catalog Code: | NB120-1870 |
| Product Name: | Cullin 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cullin 2 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody reacts with a 76kDa protein, known as Cullin 2. |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Rabbit antisera. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. |
| Localization: | Nuclear |
| Background: | Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-CUL 2 antibody, anti-MGC131970 antibody |
| Package Size: | 0.5 ml |
| Preservative: | sodium azide |
| General References: | General / background references:Michel JJ & Xiong Y Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ 9:435-49 (1998). Kipreos ET et al. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85:829-39 (1996). |
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