| CatalogCode: | NB120-18058 |
| ProductName: | Vinculin Antibody |
| Product Description: | Mouse Monoclonal anti-Vinculin (SPM227) |
| Clone: | SPM227 |
| Clonality: | Monoclonal |
| Immunogen: | Full length native protein (semi-purified) (Human). |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | IF: Use at an assay dependent dilution. IHC-P: 1/25 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.IP: Use at 2ug/mg of lysate.WB: Use at a concentration of 1 - 2 mg/ml for 2hrs at RT. Predicted molecular weight: 130kDa (vinculin) and 150kDa (meta-vinculin)Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane and Cytoplasmic |
| Control: | HeLa cells, skin. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | Preservative: Sodium AzideConstituents: BSA, 10mM PBS. pH 7.4 |
| ListPrice: | 330 |
| AppSummary: | IF, IHC-P, IP, WB |
| SpeciesSummary: | Ha, Hu |
| ALTnames: | anti-VIN-1 antibody |
| ProteinTarget: | Vinculin |
| PackageSize: | 0.5 ml |
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