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| Catalog Code: | NB120-17845 |
| Product Name: | E2F4 Antibody |
| Product Description: | Mouse Monoclonal anti-E2F4 (SPM179) |
| Clone: | SPM179 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Recombinant DP1 protein. |
| Epitope: | Not determined. |
| Cross Reactivity: | Cross-reacts with Human and Rat. |
| Packaging: | 0.5 ml protein G purified Mouse IgG. |
| Uses: | GSA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.WB: Use at a concentration of 1 - 2 ug/ml. Predicted molecular weight: 56 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Buffer: | BSA, 10mM PBS. pH 7.4 |
| Application Summary: | immunofluorescence , Western Blot , Immunohistochemistry-Paraffin , GSA, |
| Species Summary: | Hu, Rt |
| Alternate Names: | Anti-E2F 4 antibody, Anti-E2F transcription factor 4 antibody, Anti-E2F transcription factor 4 p107/p130 binding antibody, Anti-p107/p130 binding protein antibody, Anti-Transcription factor E2F 4 antibody, Anti-Transcription factor E2F4 antibody |
| Package Size: | 0.5 ml |
| Preservative: | Sodium Azide |
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more information: | company product webpage |